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modified eagle medium (mem) non-essential amino acid solution  (Thermo Fisher)


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    Thermo Fisher modified eagle medium (mem) non-essential amino acid solution
    Modified Eagle Medium (Mem) Non Essential Amino Acid Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+eagle+medium+(mem)+non-essential+amino+acid+solution/pmc11662657-79-91-98
    Average 90 stars, based on 1 article reviews
    modified eagle medium (mem) non-essential amino acid solution - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis
    Article Snippet: In brief, cells were cultured in StemPro-34 SFM with StemPro supplement (Thermo Fisher Scientific, 10639–011), supplemented with 10% FBS (Thermo Fisher Scientific, 10091–148), 40 ng/mL recombinant human glial cell line-derived neurotrophic factor (GDNF, R&D, Lot# VQ3222081), 10 ng/mL human epidermal growth factor (EGF, Thermo Fisher Scientific, PHG0311), 1000 U human leukemia inhibitory factor (LIF, Millipore, LIF1050), 2 ng/mL human basic fibroblast growth factor (bFGF, Millipore, GF003), 5 ng/mL recombinant human Wnt3a (R&D, 5036-WN-010), 2 mM L-glutamine (Sigma, 56–85–9), 1 mM β-mercaptoethanol (Sigma, M3148), 1 mM sodium pyruvate (Gibco, 11360–070), 1% Modified Eagle Medium (MEM) non-essential amino acid solution (Gibco, 11140–050) and 60 μM putrescine (Sigma, V900377).

    Article Title: SARS-CoV-2-mRNA Booster Vaccination Reverses Non-Responsiveness and Early Antibody Waning in Immunocompromised Patients – A Phase Four Study Comparing Immune Responses in Patients With Solid Cancers, Multiple Myeloma and Inflammatory Bowel Disease
    Article Snippet: Briefly, one day prior the assay, 10000 Vero cells (ATCC CCL-81TM, kindly provided by Prof. Sylvia Knapp, Medical University of Vienna, Vienna, Austria) were seeded into each well of a 96-well plate (in a Dulbecco ́s Modified Eagle ́s medium, DMEM, Gibco/Thermo Fisher, high glucose, with GlutaMAX and sodium pyruvate, supplemented with 10% fetal calf serum, FCS, Biowest, Nuaillé, France; 1% MEM non-essential amino acids solution, Gibco/Thermo Fisher; 100 U/ml penicillin and 100 μg/ml streptomycin, Gibco/Thermo Fisher).

    Cell Culture:

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100 nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 7 and 8, 50% SRM with 50% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTM media, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20 ng/ml BDNF, 20 ng/ml GDNF, 0.5 mM dibutyryl cAMP, 0.2 mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Article Title: Generation of Urine-Derived Induced Pluripotent Stem Cells and Cerebral Organoids for Modeling Down Syndrome.
    Article Snippet: Down syndrome (DS, or trisomy 21, T21), is the most common genetic cause of intellectual disability.. Alterations in the complex process of cerebral cortex development contribute to the neurological deficits in DS, although the underlying molecular and cellular mechanisms are not completely understood.. Human cerebral organoids (COs) derived from threedimensional (3D) cultures of induced pluripotent stem cells (iPSCs) provide a new avenue for gaining a better understanding of DS neuropathology.

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor.
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRMmedia with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μMPurmorphamine, and 3 μMCHIR99021; on days 7 and 8, 50% SRMwith 50% N2media supplemented with 100nM LDN193189 and 3 μMCHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTMmedia, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20ng/ml BDNF, 20 ng/ml GDNF, Nature Communications | (2023) 14:6773 13 0.5mM dibutyryl cAMP, 0.2mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Article Title: Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis.
    Article Snippet: .. In brief, cells were cultured in StemPro-34 SFM with StemPro supplement (Thermo Fisher Scientific, 10639–011), supplemented with 10% FBS (Thermo Fisher Scientific, 10091–148), 40 ng / mL D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1013/7899533 by guest on 22 N ovem ber 2024 recombinant human glial cell line-derived neurotrophic factor (GDNF, R&D, Lot# VQ3222081), 10 ng / mL human epidermal growth factor (EGF, Thermo Fisher Scientific, PHG0311), 1000 U human leukemia inhibitory factor (LIF, Millipore, LIF1050), 2 ng / mL human basic fibroblast growth factor (bFGF, Millipore, GF003), 5 ng / mL recombinant human Wnt3a (R&D, 5036-WN-010), 2 mM L-glutamine (Sigma, 56–85–9), 1 mM β-mercaptoethanol (Sigma, M3148), 1 mM sodium pyruvate (Gibco, 11360–070), 1% Modified Eagle Medium (MEM) non-essential amino acid solution (Gibco, 11140–050) and 60 μM putrescine (Sigma, V900377). .. Tree shrew Sertoli cells, treated with 10 μg / mL mitomycin C (Sigma, M4287), were used as feeder cells.

    Targeted Proteomics:

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100 nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 7 and 8, 50% SRM with 50% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTM media, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20 ng/ml BDNF, 20 ng/ml GDNF, 0.5 mM dibutyryl cAMP, 0.2 mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor.
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRMmedia with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μMPurmorphamine, and 3 μMCHIR99021; on days 7 and 8, 50% SRMwith 50% N2media supplemented with 100nM LDN193189 and 3 μMCHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTMmedia, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20ng/ml BDNF, 20 ng/ml GDNF, Nature Communications | (2023) 14:6773 13 0.5mM dibutyryl cAMP, 0.2mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Modification:

    Article Title: Erosion of X-Chromosome Inactivation in female hiPSCs is heterogeneous and persists during differentiation
    Article Snippet: .. To freeze, hiPSCs were dissociated with 0.5 mM EDTA/1x PBS and collected with Washing medium (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12-DMEM-F12 #11320-033, Gibco, 10% KnockOut TM Serum Replacement-KSR, #10828-028, Gibco, 1% MEM Non-essential Amino Acid Solution 100x-NEAA, #11140-050, Gibco), 1 mM L-Glutamine (#25030081, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol, (#31350-010, Gibco). .. After 3 minutes at 1000 rotations per minute (rpm) of centrifugation, the cells pellet was resuspended with a freezing medium composed of 90% KSR and 10% of Dimethyl Sulfoxide (DMSO, #D2438, Merck).

    Article Title: Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis.
    Article Snippet: .. In brief, cells were cultured in StemPro-34 SFM with StemPro supplement (Thermo Fisher Scientific, 10639–011), supplemented with 10% FBS (Thermo Fisher Scientific, 10091–148), 40 ng / mL D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1013/7899533 by guest on 22 N ovem ber 2024 recombinant human glial cell line-derived neurotrophic factor (GDNF, R&D, Lot# VQ3222081), 10 ng / mL human epidermal growth factor (EGF, Thermo Fisher Scientific, PHG0311), 1000 U human leukemia inhibitory factor (LIF, Millipore, LIF1050), 2 ng / mL human basic fibroblast growth factor (bFGF, Millipore, GF003), 5 ng / mL recombinant human Wnt3a (R&D, 5036-WN-010), 2 mM L-glutamine (Sigma, 56–85–9), 1 mM β-mercaptoethanol (Sigma, M3148), 1 mM sodium pyruvate (Gibco, 11360–070), 1% Modified Eagle Medium (MEM) non-essential amino acid solution (Gibco, 11140–050) and 60 μM putrescine (Sigma, V900377). .. Tree shrew Sertoli cells, treated with 10 μg / mL mitomycin C (Sigma, M4287), were used as feeder cells.

    Article Title: Deleterious Variants in ABCC12 are Detected in Idiopathic Chronic Cholestasis and Cause Intrahepatic Bile Duct Loss in Model Organisms.
    Article Snippet: Supplementary Table 2.Chemicals and Reagents Used in the Study Chemical and reagents Company Catalog number Cholic acid Sigma-Aldrich C1129-100G Hyaluronidase Sigma-Aldrich H3506 Collagenase D Sigma-Aldrich 11088882001 EpCAM, (murine); CD326, G8.8. .. DSHB Sheep anti-rat IgG Dynabeads Thermo Fisher Scientific 11035 DMEM W/F12 Gibco 11320033 MEM-NEAA Gibco 11140050 Chemically defined Lipid Concentrate Gibco 11905031 Gentamicin Gibco 15750060 MEM vitamin solution Gibco 11120052 ITS Corning Cellgro ITS MT25800CR Dexamethasone Sigma-Aldrich D4902 Mouse epidermal growth factor Peprotech 31509 Forskolin Sigma-Aldrich F3917 Triiodothyronine Sigma-Aldrich T6397 RevertAid RT Reverse Transcription Kit Thermo Fisher Scientific K1691 TaqMan Universal PCR Master Mix Thermo Fisher Scientific 4331182 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23227 ABCC12 TaqMan Probes Invitrogen Mm01241948_m1 18s Mouse TaqMan Probe Invitrogen Mm04277571 LICOR IRDye 800CW goat anti-rat IgG LI-COR Biosciences 926-32219 Donkey anti-mouse IgG secondary antibody IRDye 800CW LI-COR Biosciences 926-32212 Annexin 5 Biolegend 640906 Annexin Binding buffer Biolegend 422201 7-Aminoactinomycin D Miltenyi Biotech 130-111-568 BCA, bicinchoninic acid; DMEM, Dulbecco’s modified Eagle medium; DSHB, Developmental Studies Hybridoma Bank; EpCAM, epithelial cell adhesion molecule; ITS, insulin-transferrin-selenium; MEM, minimal essential medium; NEAA, nonessential amino acids. ..

    Knock-Out:

    Article Title: Erosion of X-Chromosome Inactivation in female hiPSCs is heterogeneous and persists during differentiation
    Article Snippet: .. To freeze, hiPSCs were dissociated with 0.5 mM EDTA/1x PBS and collected with Washing medium (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12-DMEM-F12 #11320-033, Gibco, 10% KnockOut TM Serum Replacement-KSR, #10828-028, Gibco, 1% MEM Non-essential Amino Acid Solution 100x-NEAA, #11140-050, Gibco), 1 mM L-Glutamine (#25030081, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol, (#31350-010, Gibco). .. After 3 minutes at 1000 rotations per minute (rpm) of centrifugation, the cells pellet was resuspended with a freezing medium composed of 90% KSR and 10% of Dimethyl Sulfoxide (DMSO, #D2438, Merck).

    Recombinant:

    Article Title: Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis.
    Article Snippet: .. In brief, cells were cultured in StemPro-34 SFM with StemPro supplement (Thermo Fisher Scientific, 10639–011), supplemented with 10% FBS (Thermo Fisher Scientific, 10091–148), 40 ng / mL D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1013/7899533 by guest on 22 N ovem ber 2024 recombinant human glial cell line-derived neurotrophic factor (GDNF, R&D, Lot# VQ3222081), 10 ng / mL human epidermal growth factor (EGF, Thermo Fisher Scientific, PHG0311), 1000 U human leukemia inhibitory factor (LIF, Millipore, LIF1050), 2 ng / mL human basic fibroblast growth factor (bFGF, Millipore, GF003), 5 ng / mL recombinant human Wnt3a (R&D, 5036-WN-010), 2 mM L-glutamine (Sigma, 56–85–9), 1 mM β-mercaptoethanol (Sigma, M3148), 1 mM sodium pyruvate (Gibco, 11360–070), 1% Modified Eagle Medium (MEM) non-essential amino acid solution (Gibco, 11140–050) and 60 μM putrescine (Sigma, V900377). .. Tree shrew Sertoli cells, treated with 10 μg / mL mitomycin C (Sigma, M4287), were used as feeder cells.

    Reverse Transcription:

    Article Title: Deleterious Variants in ABCC12 are Detected in Idiopathic Chronic Cholestasis and Cause Intrahepatic Bile Duct Loss in Model Organisms.
    Article Snippet: Supplementary Table 2.Chemicals and Reagents Used in the Study Chemical and reagents Company Catalog number Cholic acid Sigma-Aldrich C1129-100G Hyaluronidase Sigma-Aldrich H3506 Collagenase D Sigma-Aldrich 11088882001 EpCAM, (murine); CD326, G8.8. .. DSHB Sheep anti-rat IgG Dynabeads Thermo Fisher Scientific 11035 DMEM W/F12 Gibco 11320033 MEM-NEAA Gibco 11140050 Chemically defined Lipid Concentrate Gibco 11905031 Gentamicin Gibco 15750060 MEM vitamin solution Gibco 11120052 ITS Corning Cellgro ITS MT25800CR Dexamethasone Sigma-Aldrich D4902 Mouse epidermal growth factor Peprotech 31509 Forskolin Sigma-Aldrich F3917 Triiodothyronine Sigma-Aldrich T6397 RevertAid RT Reverse Transcription Kit Thermo Fisher Scientific K1691 TaqMan Universal PCR Master Mix Thermo Fisher Scientific 4331182 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23227 ABCC12 TaqMan Probes Invitrogen Mm01241948_m1 18s Mouse TaqMan Probe Invitrogen Mm04277571 LICOR IRDye 800CW goat anti-rat IgG LI-COR Biosciences 926-32219 Donkey anti-mouse IgG secondary antibody IRDye 800CW LI-COR Biosciences 926-32212 Annexin 5 Biolegend 640906 Annexin Binding buffer Biolegend 422201 7-Aminoactinomycin D Miltenyi Biotech 130-111-568 BCA, bicinchoninic acid; DMEM, Dulbecco’s modified Eagle medium; DSHB, Developmental Studies Hybridoma Bank; EpCAM, epithelial cell adhesion molecule; ITS, insulin-transferrin-selenium; MEM, minimal essential medium; NEAA, nonessential amino acids. ..

    Polymerase Chain Reaction:

    Article Title: Deleterious Variants in ABCC12 are Detected in Idiopathic Chronic Cholestasis and Cause Intrahepatic Bile Duct Loss in Model Organisms.
    Article Snippet: Supplementary Table 2.Chemicals and Reagents Used in the Study Chemical and reagents Company Catalog number Cholic acid Sigma-Aldrich C1129-100G Hyaluronidase Sigma-Aldrich H3506 Collagenase D Sigma-Aldrich 11088882001 EpCAM, (murine); CD326, G8.8. .. DSHB Sheep anti-rat IgG Dynabeads Thermo Fisher Scientific 11035 DMEM W/F12 Gibco 11320033 MEM-NEAA Gibco 11140050 Chemically defined Lipid Concentrate Gibco 11905031 Gentamicin Gibco 15750060 MEM vitamin solution Gibco 11120052 ITS Corning Cellgro ITS MT25800CR Dexamethasone Sigma-Aldrich D4902 Mouse epidermal growth factor Peprotech 31509 Forskolin Sigma-Aldrich F3917 Triiodothyronine Sigma-Aldrich T6397 RevertAid RT Reverse Transcription Kit Thermo Fisher Scientific K1691 TaqMan Universal PCR Master Mix Thermo Fisher Scientific 4331182 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23227 ABCC12 TaqMan Probes Invitrogen Mm01241948_m1 18s Mouse TaqMan Probe Invitrogen Mm04277571 LICOR IRDye 800CW goat anti-rat IgG LI-COR Biosciences 926-32219 Donkey anti-mouse IgG secondary antibody IRDye 800CW LI-COR Biosciences 926-32212 Annexin 5 Biolegend 640906 Annexin Binding buffer Biolegend 422201 7-Aminoactinomycin D Miltenyi Biotech 130-111-568 BCA, bicinchoninic acid; DMEM, Dulbecco’s modified Eagle medium; DSHB, Developmental Studies Hybridoma Bank; EpCAM, epithelial cell adhesion molecule; ITS, insulin-transferrin-selenium; MEM, minimal essential medium; NEAA, nonessential amino acids. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Deleterious Variants in ABCC12 are Detected in Idiopathic Chronic Cholestasis and Cause Intrahepatic Bile Duct Loss in Model Organisms.
    Article Snippet: Supplementary Table 2.Chemicals and Reagents Used in the Study Chemical and reagents Company Catalog number Cholic acid Sigma-Aldrich C1129-100G Hyaluronidase Sigma-Aldrich H3506 Collagenase D Sigma-Aldrich 11088882001 EpCAM, (murine); CD326, G8.8. .. DSHB Sheep anti-rat IgG Dynabeads Thermo Fisher Scientific 11035 DMEM W/F12 Gibco 11320033 MEM-NEAA Gibco 11140050 Chemically defined Lipid Concentrate Gibco 11905031 Gentamicin Gibco 15750060 MEM vitamin solution Gibco 11120052 ITS Corning Cellgro ITS MT25800CR Dexamethasone Sigma-Aldrich D4902 Mouse epidermal growth factor Peprotech 31509 Forskolin Sigma-Aldrich F3917 Triiodothyronine Sigma-Aldrich T6397 RevertAid RT Reverse Transcription Kit Thermo Fisher Scientific K1691 TaqMan Universal PCR Master Mix Thermo Fisher Scientific 4331182 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23227 ABCC12 TaqMan Probes Invitrogen Mm01241948_m1 18s Mouse TaqMan Probe Invitrogen Mm04277571 LICOR IRDye 800CW goat anti-rat IgG LI-COR Biosciences 926-32219 Donkey anti-mouse IgG secondary antibody IRDye 800CW LI-COR Biosciences 926-32212 Annexin 5 Biolegend 640906 Annexin Binding buffer Biolegend 422201 7-Aminoactinomycin D Miltenyi Biotech 130-111-568 BCA, bicinchoninic acid; DMEM, Dulbecco’s modified Eagle medium; DSHB, Developmental Studies Hybridoma Bank; EpCAM, epithelial cell adhesion molecule; ITS, insulin-transferrin-selenium; MEM, minimal essential medium; NEAA, nonessential amino acids. ..

    Binding Assay:

    Article Title: Deleterious Variants in ABCC12 are Detected in Idiopathic Chronic Cholestasis and Cause Intrahepatic Bile Duct Loss in Model Organisms.
    Article Snippet: Supplementary Table 2.Chemicals and Reagents Used in the Study Chemical and reagents Company Catalog number Cholic acid Sigma-Aldrich C1129-100G Hyaluronidase Sigma-Aldrich H3506 Collagenase D Sigma-Aldrich 11088882001 EpCAM, (murine); CD326, G8.8. .. DSHB Sheep anti-rat IgG Dynabeads Thermo Fisher Scientific 11035 DMEM W/F12 Gibco 11320033 MEM-NEAA Gibco 11140050 Chemically defined Lipid Concentrate Gibco 11905031 Gentamicin Gibco 15750060 MEM vitamin solution Gibco 11120052 ITS Corning Cellgro ITS MT25800CR Dexamethasone Sigma-Aldrich D4902 Mouse epidermal growth factor Peprotech 31509 Forskolin Sigma-Aldrich F3917 Triiodothyronine Sigma-Aldrich T6397 RevertAid RT Reverse Transcription Kit Thermo Fisher Scientific K1691 TaqMan Universal PCR Master Mix Thermo Fisher Scientific 4331182 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23227 ABCC12 TaqMan Probes Invitrogen Mm01241948_m1 18s Mouse TaqMan Probe Invitrogen Mm04277571 LICOR IRDye 800CW goat anti-rat IgG LI-COR Biosciences 926-32219 Donkey anti-mouse IgG secondary antibody IRDye 800CW LI-COR Biosciences 926-32212 Annexin 5 Biolegend 640906 Annexin Binding buffer Biolegend 422201 7-Aminoactinomycin D Miltenyi Biotech 130-111-568 BCA, bicinchoninic acid; DMEM, Dulbecco’s modified Eagle medium; DSHB, Developmental Studies Hybridoma Bank; EpCAM, epithelial cell adhesion molecule; ITS, insulin-transferrin-selenium; MEM, minimal essential medium; NEAA, nonessential amino acids. ..



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